ab 329956  (Cell Signaling Technology Inc)

Journal: Biochemical and biophysical research communications

Article Title: Synthetic retinoid CD437 induces apoptosis and acts synergistically with TRAIL receptor-2 agonist in malignant melanoma.

doi: 10.1016/j.bbrc.2012.03.023

Figure Lengend Snippet: Fig. 2. Cell death induced by CD437. FEMX-1 and WM239 cells were treated with 1 lM CD437 and harvested after the indicated time. (A) Dead/dying cells were stained with trypan blue and both living and dead cells were counted. Dead cells are presented as percentage of total cell count (Diamonds: control cells and treated cells: squares). (B) Protein expressions of Caspase-8, -9, -3, PARP and DFF45 were examined by western blot analysis. a-Tubulin has been used as loading control. Data are the mean or representative of at least three independent experiments.

Article Snippet: Primary antibodies Caspase 3 (#9662/#9664 (even mix)), Caspase 8 (#9746), Caspase 9 (#9502), DFF45 (#9732), cleaved DFF45 (#9731), p21 (#2946) and PARP (#9532), were purchased from Cell Signaling (Beverly, MA). a-Tubulin (DMIB) was from Calbiochem (Nottingham, UK), whereas cathepsin D (sc-6486) and p53 (sc126) were obtained from Santa Cruz (Santa Cruz, CA).

Techniques: Staining, Cell Counting, Control, Western Blot